Propolis is a resinous compound gathered by bees from tree buds and other botanical sources, utilized to seal and secure their hives. Known for its antimicrobial homes, propolis has actually been used in standard medication to support injury recovery, improve the body immune system, and deal with different disorders.
A brand-new research study analyzed how artepillin C effects both healthy and malignant cells, together with examining how modifications in the pH of the medium affect its efficiency.
Propolis has actually long been utilized in standard medication and has actually won attention from the clinical neighborhood following evidence of its health advantages, that include antioxidant, anti-inflammatory, antimicrobial, antitumor, and immunomodulatory homes.
Its structure differs according to origin, geographical place, and the bee < period class ="glossaryLink" aria-describedby ="tt" data-cmtooltip ="<div class=glossaryItemTitle>species</div><div class=glossaryItemBody>A species is a group of living organisms that share a set of common characteristics and are able to breed and produce fertile offspring. The concept of a species is important in biology as it is used to classify and organize the diversity of life. There are different ways to define a species, but the most widely accepted one is the biological species concept, which defines a species as a group of organisms that can interbreed and produce viable offspring in nature. This definition is widely used in evolutionary biology and ecology to identify and classify living organisms.</div>" data-gt-translate-attributes=" [{"attribute":"data-cmtooltip", "format":"html"}]" tabindex ="0" function ="link" > types that produces it. Researchers connected with SãoPauloStateUniversity( UNESP) inBrazil and theUniversity ofSouthernDenmark (SDU) set out to evaluateBrazilian green propolis, which is produced by theAfricanized honeybee((***************** )Apis mellifera).
Its primary part is artepillin C( 3,5-diprenyl-4-hydroxycinnamic < period class ="glossaryLink" aria-describedby ="tt" data-cmtooltip ="<div class=glossaryItemTitle>acid</div><div class=glossaryItemBody>Any substance that when dissolved in water, gives a pH less than 7.0, or donates a hydrogen ion.</div>" data-gt-translate-attributes="[{"attribute":"data-cmtooltip", "format":"html"}]" tabindex ="0" function ="link" > acid ), a phenolic substance mainly discovered in the resin of Baccharis dracunculifolia , a nativeBrazilian plant (popular name alecrim-do-campo) understood to have antitumor homes.
“Prior research showed that artepillin C can alter model biological membranes, thin films around living cells, especially when we vary the pH of the medium in which they are placed,” statedWallanceMoreiraPazin, a teacher in theDepartment ofPhysics andMeteorology at UNESP’sBauruSchool ofSciences (FC).
Study on Artepillin C’sEffects on(*************************************************************************************************************** )
The scientists chose to discover how healthy cells and growth cells acted biochemically when brought into contact with artepillin C, focusing for this function on fibroblasts– the main cells in recovery and upkeep of connective tissue– and glioblastoma cells respectively. Glioblastoma is the most typical main brain cancer.
The culture medium’s pH was likewise differed to see whether a more acidic microenvironment would result in various impacts of artepillin C. “This is relevant because tumor tissue converts glucose into lactic acid and makes the extracellular microenvironment more acid,” stated Pazin, very first author of a post on the research study released in the journal Life
They next carried out a careful analysis of the impacts of the propolis on cell membranes, utilizing an optical microscopic lense to observe the stability, fluidity, and morphology of the membranes. The analysis revealed that artepillin C engaged extremely with growth cells, modifying their fluidity and capacity for reorganization. It likewise activated autophagy, a cleaning procedure that includes the deterioration of used, unusual, or malfunctioning cellular elements.
The research study was supported by FAPESP by means of 4 tasks (16/09633 -4, 17/23426 -4, 18/22214 -6 and 20/12129 -1). According to Pazin, it adds to a much deeper understanding of the compound’s action systems and supplies insights for future research study resulting in ingenious treatments for cancer.
“However, although in vitro trials have demonstrated high efficiency for this molecule’s biological activities, oral or topical administration to patients would be hindered by certain particularities, such as low absorption and bioavailability,” Pazin stated. “In this context, strategies to enhance its therapeutic action will be required in order for progress to be possible in the use of artepillin C against tumors. An example would be the deployment of nanocarriers for controlled release.”
Reference: “pH-Dependence Cytotoxicity Evaluation of Artepillin C against Tumor Cells” by Wallance M. Pazin, Renata R. Miranda, Karina A. Toledo, Frank Kjeldsen, Carlos J. L. Constantino and Jonathan R. Brewer, 8 November 2023, Life
DOI: 10.3390/ life13112186
