Fatty acids and 3-hydroxypropionic acid were effectively produced by engineering co-utilization of glucose and xylose in Ogataea polymorpha for lignocellulose biorefinery. Credit: DICP
A group from the Dalian Institute of Chemical Physics has actually originated a microbial system that enhances the production of important chemicals from lignocellulosic biomass by boosting sugar co-fermentation.
Lignocellulosic biomass is an eco-friendly feedstock for 2 nd– generation biomanufacturing. In specific, effective co-fermentation of blended glucose and xylose in lignocellulosic hydrolysates is an essential concern in decreasing item expenses.
However, co-utilization of xylose and glucose in microorganisms is challenging due to restricted xylose assimilation and the glucose repression result.
Recent Advances by DICP Researchers
Recently, a research study group led byProf Yongjin Zhou from the Dalian Institute of Chemical Physics (DICP) of the Chinese Academy of Sciences (CAS) has actually proposed a microbial platform for lignocellulose bio-refinery. It can effectively manufacture acetyl-CoA derivatives, such as fats (FFA) and 3-hydroxypropionic < period class =(***************************************************** )aria-describedby ="tt" data-cmtooltip ="<div class=glossaryItemTitle>acid</div><div class=glossaryItemBody>Any substance that when dissolved in water, gives a pH less than 7.0, or donates a hydrogen ion.</div>" data-gt-translate-attributes="[{"attribute":"data-cmtooltip", "format":"html"}]" > acid( 3-HP), owing to the improved supply of precursor acetyl-CoA and cofactor NADPH by rewiring the cellular metabolic process ofOgataea(Hansenula) polymorpha
(************** )This research study will be released today(August24) inNatureChemicalBiology
Technological Innovations andResults(********************** )
The scientists effectively attained the synchronised usage of glucose and xylose.(**************************************************************************************** )did this by presenting a hexose transporter mutant and xylose isomerase, and overexpressing the native xylulokinase to boost xylose catabolism and import.
The crafted stress produced 7.0 g/L FFA from genuine lignocellulosic hydrolysates in shake flasks and 38.2 g/L FFA from simulated lignocellulose in a bioreactor. Furthermore, this exceptional cell factory was broadened for 3-HP production by a metabolic changing technique, getting the greatest 3-HP titer of 79.6 g/L from simulated lignocellulose.
“Our work recognized co-utilization of xylose and glucose without jeopardizing native glucose metabolic process and showed the capacity of O. polymorpha as a cell factory to produce flexible value-added chemicals from lignocellulose,” statedProf Zhou.
Reference: “Engineering co-utilization of glucose and xylose for chemical overproduction from lignocellulose” 24 August 2023, Nature Chemical Biology
DOI: 10.1038/ s41589-023-01402 -6
